Composite

Part:BBa_K1651002:Design

Designed by: YANG YUANZHAN   Group: iGEM15_BIT   (2015-09-13)


LasI driven by pBAD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1900
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1462
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

This is an circuit which could be activated by arabinose to produce LasI. It ia an component of our amplifier.


Source

Series group effect element from P.aeruginosa.

References